059492
Tubulin, beta (β-monoE)
Size
£620.00
- SKU:
- 059492
- Purity:
- Purified by Immunoaffinity chromatography. ≥95% (SDS-PAGE)
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to D431EQGEFE(E-COOH*)EEEG441-NH2 of human tubulin β-2A chain (*secondary glutamate branched from γ-carboxyl group of glutamate as isopeptide bond).
- Format:
- Supplied as a liquid in PBS, 0.02% Proclin 300.
- Extra Details:
- Polyglutamylation is an evolutionarily conserved protein post-translational modification in which glutamate side chains of variable lengths are formed on the modified protein. The most prominent substrate is tubulin, the building block of microtubules (MTs). Polyglutamylation has been found on both, α- and β-tubulin. It is catalyzed by multiple enzymes that belong to the family of tubulin tyrosine-ligase like (TTLL) enzymes. These TTLLs have substrate (α- vs β-tubulin) as well as reaction (initiation vs elongation) specificities. These subtle changes in levels of polyglutamylation on specific tubulin isoforms could in turn be influencing the diverse MT functions that are regulated by polyglutamylation. The β-monoE antibody selectively labels glutamylation of β-tubulin due to its specificity to a sequence motif (GE(*-E)F) that only exists in β-tubulin isotypes. It allows to specifically detect β-tubulin glutamylation in brain tubulin, where other antibodies predominantly detect α-tubulin glutamylation. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:2000 Immunocytochemistry: 1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice