U1-70K (Small Nuclear Ribonucleoprotein 70kDa (U1), U1 Small Nuclear Ribonucleoprotein 70 kDa, small Nuclear Ribonucleoprotein 70kDa (RNP antigen), U1 snRNP 70 kDa)

Size

£843.00

SKU:: 059480
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to C-terminal domain of human U1-70K.
Clone:: 9C4.1
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: U1 small nuclear ribonucleoprotein 70 kDa (U1-70K) is one of three specific proteins found within the U1 snRNP core, which includes at least seven core proteins found in all U snRNPs and three specific proteins; U1-70K, U1-A, and U1-C. U1-70K is involved in mediating pre-mRNA splicing through binding the loop I region of U1-snRNA with its RPM (RNA recognition motif) binding domain. In addition, its RNA binding domain, U1-70K also contains two argine-rich regions, accented with acidic or serine residues, that is thought to have trans-acting functions. Alternative splicing results in 4 known isoforms. Truncated forms of U1-70K are unable to bind RNA. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Immunocytochemistry: 1:500 has been shown to detect U1-70K in NIH/3T3, A431, and HeLa cells. Western Blot: 0.5ug/ml detected U1-70K on 10ug of HeLa cell lysate. Optimal dilutions to be determined by the researcher. Control: HeLa cell lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice