059479
SFTPC (Pulmonary Surfactant-Associated protein C, SP-C, Pulmonary Surfactant-Associated Proteolipid SPL(Val), SP5)
Size
£882.00
- SKU:
- 059479
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- KLH-conjugated linear peptide corresponding to the N-terminus of human SFTPC.
- Format:
- Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
- Extra Details:
- SFTPC, also known as SFTP2 and Pulmonary surfactant-associated protein C, is a complex mixture of phospholipids and proteins secreted from airway epithelial cells (type II cells) in alveoli which reduces the surface tension at the alveolar air liquid interface, providing alveolar stability necessary for normal ventilation. Four distinct proteins isolated from pulmonary surfactant are termed surfactant proteins A, B, C, and D. SP A (28-36kD) and SP D (43kD) are collagenous carbohydrate binding proteins, whereas SP B (8-9kD) and SP C (4kD) are non collagenous hydrophobic proteins. SP C is a fragment of a much larger precursor protein of 21kD. The precursor contains an exceedingly hydrophobic region of 34 amino acids that comprises most of the mature SP C, and a unique poly-valine domain. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (FFPE): 1:500 detected SFTPC in human lung tissue. Western Blot: 1ug/ml detected SFTPC in 10ug of rat lung tissue lysate. Optimal dilutions to be determined by the researcher. Control: Rat lung tissue lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice