059473
Puromycin
Size
£881.00
- SKU:
- 059473
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Puromycin from Streptomyces alboniger
- Clone:
- 12D10
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
- Extra Details:
- Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3′ end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells. Puromycin is incorporated in neosynthesized proteins. In the presense of Puromycin only, this antibody detects Puromycin-incorporated neosynthesized proteins at multiple molecular weights. However, a weaker signal is observed in the co-presense of Cycloheximide, an inhibitor of protein biosynthesis in eukaryotic organisms. 059473 detects puromycin incorporated into protein. Monoclonal antibodies to puromycin may be used with standard immunochemical methods. Applications: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Immunocytochemistry and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:25,000 detected Puromycin-incorporated neosynthesized proteins in HEK293 cell lysates treated with Puromycin only. This antibody also detected small mounts of Puromycin-incorporated neosynthesized proteins in HEK293 cells treated with Puromycin and Cyclohexamide. Immunocytochemistry: 1:10,000 detected Puromycin-incorporated neosynthesized proteins in HeLa cells treated with Puromycin. Immunohistochemistry: detects Puromycin-incorporated neosynthesized protein (Goodman, C. A., et al. (2010). FASEB J. 25(3):1028-1039.). Optimal dilutions to be determined by the researcher. Control: HEK293 cell lysates treated with Puromycin and Cyclohexamide, or with Puromycin only. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice