059471
Estrogen Receptor alpha
Size
£850.00
- SKU:
- 059471
- Purity:
- Ascites
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Linear peptide corresponding to human Estrogen Receptor α. Epitope: unknown
- Clone:
- F3-A
- Format:
- Supplied as a liquid. No preservative added.
- Extra Details:
- Estrogen Receptor α (ERα) is a 65kD nuclear hormone receptor. This steroid hormone receptor is involved in the regulation of eukaryotic gene expression and affects cellular proliferation and differentiation in target tissues. ERα is a ligand-activated transcription factor and upon being bound by a ligand such as estrogen, a conformational change occurs that allows dimerization and binding to an estrogen response element. When bound to DNA, Estrogen Receptor α can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Two ERs are known, ERα, classically known as ER, and ERβ. The Estrogen Receptor is believed to play an important role in the growth and development of a subset of hormone dependent human breast cancers. ERβ is expressed in prostate, ovary, and other reproductive organs. Although its steroid specificity is very similar to that of the ERα, the molecular mechanisms regulating the transcriptional activity of ERβ may be distinct from those regulating ERα. Applications: Suitable for use in Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 detected Estrogen Receptor α on 10ug of mouse ovary tissue lysate. Immunocytochemistry: 1:500 detected Estrogen Receptor α in A431 cells. Optimal dilutions to be determined by the researcher. Control: Mouse ovary tissue lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice