059470
Estrogen Receptor alpha
Size
£832.00
- SKU:
- 059470
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of rat Estrogen Receptor alpha. Species Sequence Homology: mouse
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
- Extra Details:
- Estrogen receptor (UniProt: P06211; also known as ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1) is encoded by the Esr1 (also known as Esr, Estr, Nr3a1) gene (Gene ID: 24890) in rat. Estrogen receptor α is a member of the steroid family of nuclear receptors; a critical nuclear receptor that mediates the physiological effects of estrogens. Estrogen receptor α contains several functional domains, including a ligand-binding domain, DNA-binding domain, and activation functions (AF-1 and AF-2), which regulate transcriptional activity. It is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor α can positively or negatively regulate gene transcription. Estrogen receptor α is expressed in various tissues, including the brain, breast, liver, the cardiovascular system, uterus, and placenta, where it plays roles in development, reproduction, and metabolic regulation. Estrogen receptor α both a nuclear receptor as well as a membrane-associated receptor through post-translational modifications like palmitoylation. This dual localization enables Estrogen receptor α to participate in both genomic and non-genomic signaling pathways, such as interactions with the PI3K/AKT and MAPK/ERK pathways, and activation of endothelial nitric oxide synthase (eNOS). Protein-protein interactions involving Estrogen receptor α include binding to transcription factors like AP-1 and SP-1, as well as interaction with caveolin-1 at the membrane. Dysregulation of Estrogen receptor α signaling is implicated in various diseases, including hormone-dependent cancers (e.g., breast cancer) and metabolic disorders. Applications: Suitable for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 detected Estrogen receptor α in MCF7 cell lysate. Immunohistochemistry (FFPE): 1:1000 detected Estrogen receptor α in human breast cancer and uterine cancer tissue sections and a 1:500 dilution in human cervical cancer tissue sections using heat-induced epitope retrieval (HIER). Immunocytochemistry: 1:500 detected Estrogen Receptor α in MCF7 cells. Immunoprecipitation: 4ug immunoprecipitated Estrogen receptor α from MCF7 cell lysate. Optimal dilutions to be determined by the researcher. Control: MCF7 cell lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice