Albumin, Human Serum, Glycated (HSA)

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£639.00

SKU:: 059469
Storage Conditions:: -20°C
Supplier:: United States Biological
CAS Number:: 70024-90-7
Extra Details:: Albumin gene is mapped to human chromosome 4q13.3. It is synthesized as a preproalbumin with 609 amino acids in the liver. The mature albumin comprising 585 residues, is generated by the cleavage of signal peptide. Albumin is the major circulating protein in serum. It comprises three domains (I, II and III), each of which is subdivided into two subdomains A and B. A total of six ligand binding site is associated with albumin. Domain IIA and IIIA harbor small heterocyclic or aromatic compounds binding site. Long-chain fatty acid binding sites is present in IB and IIIB domains. Albumin is crucial for physiological processes and is the major transporter for lipids, metal ions and amino acids. It has antioxidant functionality and binds to drugs as well. Mutation in the albumin gene is implicated in an autosomal recessive disorder called Analbuminemia, which is characterized by low levels of albumin. The major glycation site is lysine 525 in albumin. Glycated albumin is a marker of glycemia in diabetes mellitus patients and is useful in assessment of HbA1c based glycemic control. Naturally occurring glycated albumin is a sensitive indicator of glycemic control by diabetic patients. Albumin, glycated human has been used as a component of serum-free medium for retinal microglia activation to stimulate immortalized proximal tubule epithelial (HK-2) cells for fibronectin production as medium supplement in human retinal microvascular endothelial cells (HRMECs) cultures to test its effect on family with sequence similarity 18, member B (FAM18B) expression. Source: Human serum, glycated in vitro. CAS No: 70024-90-7 Concentration: 90-100% protein (Biuret) Extent of Labeling: 1-5mol hexose (as fructosamine)/mol albumin. Form: Supplied as an off-white lyophilized powder containing citrate buffer salts. Solubility (Turbidity): 10mg/ml, H20 Application: Suitable for use in cell culture. Other applications not tested. Storage and Stabiltiy: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS or ddH2O. Do NOT vortex. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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