CD3 (APC)

Size

£672.00

SKU:: 059447
Application:: Flow Cytometry
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rat
Immunogen:: T cell hybridoma D1
Clone:: 17A2
Format:: Supplied as a liquid in PBS, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Extra Details:: CD3 a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28kD and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 cells using mouse splenocytes. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.