AAV, pan (Intact Particle)

Size

£889.00

SKU:: 059431
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Human
Immunogen:: AAV capsids
Clone:: 1C4H (recomb)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide.
Extra Details:: This anti-pan AAV recombinant human antibody specifically recognizes a conformational epitope only present on fully assembled and intact AAV capsids. It does not detect denatured AAV capsids, making it a versatile tool for various applications, such as tracking the localization of viral particles, studying protein-protein interactions, facilitating AAV particle purification, and determining total AAV capsid titers. Additionally, the antibody exhibits neutralizing activity against multiple AAV serotypes. Demonstrates broad binding capabilities across all AAV serotypes, with the exception of AAV12. Furthermore, it binds to numerous additional AAV variants, underscoring its versatility in diverse research and quality control settings. Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, Neutralization, Dot Blot and ELISA. Other applications not tested. Recommended Dilutions: Immunofluorescence (IC): 1:20-1:50 (1-2.5ug/ml). Dot Blot: 1:500 (0.1ug/ml; non-denaturing conditions). Neutralization: EC50 ~7ng/ml (AAV1), ~195ng/ml (AAV3), ~248ng/ml (AAV5), ~8ng/ml (AAV6), ~52ng/ml (AAV8), ~27ng/ml (AAV9), ~441ng/ml (AAVrh10), assay dependent. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice