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059414

MHC Class 2, I-A/I-E (APC)

Size

£563.00

SKU:
059414
Application:
Flow Cytometry
Purity:
Purified by affinity chromatography.
Storage Conditions:
4°C Do Not Freeze
Supplier:
United States Biological
Host:
Rat
Immunogen:
Activated C57BL/6 mouse spleen cells
Clone:
M5/114.15.2
Format:
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Extra Details:
These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: ≤0.25ug/10e6 cells in 100ul volume. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Shipping Conditions:
Blue Ice