CLEC9A (C-type Lectin-like Receptor 9, CD370)

Size

£895.00

SKU:: 059391
Application:: IHC-Fr
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to Lys57-Ile264 from mouse CLEC9a, expressed in CHO cells.
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: CLEC9a (C-type lectin domain family member A; also DNGR-1) is a 50-52kD member of the group V C-type lectin domain containing family of receptors. In mouse, it is an activation receptor expressed on both CD8 alpha + CD24+ and plasmacytoid dendritic cells (DC). It assists in endocytosis but not phagocytosis, and appears to induce cytotoxic antitumor T cells by participating in class I MHC antigen presentation. CD8 alpha + DCs are believed to be particularly important for the disposal of dead cell material. Mouse CLEC9a is a 264 amino acid (aa) type II transmembrane glycoprotein. It contains a 35 aa cytoplasmic segment (aa1-35) and a 208 aa extracellular region (aa57-264) that possesses one C-type lectin domain (aa144-256). CLEC9a forms disulfide-linked homodimers on the cell surface. There are multiple splice variants. The CLEC9a SwissProt entry (Q8BRU4) is 238 aa in length and shows a deletion of aa106-131. Other isoforms contain either a 43 or a 67 aa substitution for aa106-264, a Val substitution for 31-58, and a combination of the just mentioned Val substitution coupled to a deletion of aa106-131. Both rat and human CLEC9a appear to be absent aa106-131 found in full-length mouse CLEC9a. Taking this into account, over aa57-264, mouse CLEC9a shares 50% and 70% aa identity with human and rat CLEC9a, respectively. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 5-15ug/ml with perfusion fixed frozen sections of mouse spleen. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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