Sodium Pump Subunit alpha-2 (APC)

Size

£1,042.00

SKU:: 059388-APC
Purity:: Purified by affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: KLH-conjugated linear peptide corresponding to rat Sodium Pump Subunit alpha-2 at the second cytoplasmic domain. Species Sequence Homology: porcine and bovine (100%).
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Extra Details:: Sodium/potassium-transporting ATPase subunit alpha-2 (EC 3.6.3.9; UniProt P06686; also known as Na+/K+ -ATPase alpha 2 subunit, Na(+)/K(+) ATPase alpha(+) subunit, Sodium pump subunit alpha-2) is encoded by the Atp1a2 (also known as RATATPA2) gene (Gene ID 24212) in rat. Mammalian Na+/K+ ATPase is a heteromultimeric complex composed of alpha, beta, and gamma subunits. The alpha subunit catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ across the plasma membrane. The alpha subunit contains ten transmembrane domains with both its N- and C-termini in the cytoplasm. More than 50 mutations have been identified affecting the human ATP1A2 and ATP1A3 genes that are known to cause rapid-onset Dystonia Parkinsonism, familial hemiplegic migraine, alternating hemiplegia of childhood, and variants of familial hemiplegic migraine with neurological complications including seizures and various mood disorders. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunohistochemistry (FFPE): Requires HIER using sodium citrate buffer pH 6.0 prior to staining. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

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