Myelin Proteolipid Protein

Size

£800.00

SKU:: 059378
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Purified Myelin proteolipid protein from bovine brain.
Clone:: AA3
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Extra Details:: Myelin proteolipid protein (UniProt: P04116; also known as PLP, DM20, Lipophilin) is encoded by the PLP1 (also known as PLP) gene (Gene ID: 281410) in bovine. Myelin proteolipid protein is a highly conserved, multi-pass membrane protein with three cytoplasmic domains, four transmembrane domains, and two extracellular domains. It is composed of a series of alternating hydrophobic and hydrophilic domains. It is the major myelin protein from the central nervous system and plays an important role in the formation or maintenance of the multilamellar structure of myelin. It is shown to be the most abundant protein of the central nervous system (CNS) myelin sheath with a relative abundance of about 17% of the myelin proteins. It is mainly expressed by myelin forming oligodendrocytes in the CNS and Schwann cells in the peripheral nervous system. PLP1 gene is reported to encode two isoforms; a 30kD PLP and a 25kD DM20 that has a deletion of 35 amino acids in the second cytoplasmic domain. The DM20 protein is expressed prior to PLP during development and as development proceeds PLP becomes the more predominant protein. Although the molecular mass of PLP is ~ 30kD, it migrates anomalously on SDS gels and gives a lower apparent molecular mass. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 detected Myelin Proteolipid Protein in HepG2 cell lysate. Immunohistochemistry (FFPE): 1:50 detected Myelin Proteolipid Protein in human cerebral cortex and human cerebellum tissue sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice