059364
CAT (Chloramphenicol Acetyltransferase)
Size
£639.00
- SKU:
- 059364
- Purity:
- Ascites
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Chloramphenicol acetyl transferase
- Clone:
- CAT-1
- Format:
- Supplied as a liquid.
- Extra Details:
- Chloramphenicol acetyltransferase (UniProt: P62577; also known as EC:2.3.1.28, CAT) is encoded by the Cat gene (Gene ID: 59683728) in E. coli. Chloramphenicol acetyltransferase is an enzyme that catalyzes the acetyl-S-CoA-dependent acetylation of chloramphenicol at the 3-hydroxyl group leading to the production of 3-acetoxy chloramphenicol that does not bind to bacterial ribosomes and does not inhibit peptidyltransferase activity. It displays high stability in cells and has a half-life of about 48 hours. A highly reactive histidine residue has been implicated in the catalytic mechanism. CAT is shown to be an effector of chloramphenicol resistance in bacteria. Its synthesis is shown to be constitutive in E. coli and other Gram-negative bacteria that harbor plasmids bearing the structural gene for the enzyme. However, in Gram-positive bacteria such as staphylococci and streptococci it is synthesized only in the presence of chloramphenicol and related compounds. Applications: Suitable for use in Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 1:400, 4% PFA fixed cells (5-10′ RT, block with 3% normal secondary serum in PBS with 0.1% triton X-100 to permeabilize cell membrane (1hr). Western Blot: 1:2000-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice