IFNGR1, Extracellular Ligand-binding Domain, Recombinant, Human, His-Tag, aa1-245

Size

£734.00

SKU:: 058643
Application:: WB
Molecular Weight:: 27.3
Purity:: ≥97% (SDS-PAGE)
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as lyophilized powder from PBS, pH 7.4. Reconstitute with sterile ddH2O.
Extra Details:: The interferon-γ (IFN-γ) receptor is a heterodimeric receptor with roles in innate and adaptive immunity, as well as a wide range of infectious and inflammatory diseases. It is composed of two IFNGR1 chains that bind IFN-γ and two non-ligand binding IFNGR2 chains that facilitate signal transduction. IFNGR1 is a transmembrane protein that is composed of an extracellular IFN-γ binding domain and an intracellular domain that contains binding motifs for JAK1 and the transcription factor STAT1, and an isoleucine-leucine sequence at residues 270 and 271 that is required for receptor internalization. Upon INF-γ binding, IFNGR1 is phosphorylated by JAK1 to induce STAT1 recruitment to the receptor, which is then translocated to the nucleus to initiate gene transcription. Ifngr1-/- mice exhibit increased susceptibility to bacterial, parasitic, and viral infections, such as Theiler's murine encephalomyelitis, L. major, L. monocytogenes, and various mycobacteria. IFNGR1 SNPs have been associated with both increased and decreased susceptibility to pulmonary tuberculosis infection in humans. IFNGR1 SNPs have also been associated with increased susceptibility to H. pylori infection and increased risk of developing chronic gastritis and gastric carcinomas. This protein consists of 239 amino acids after removal of the signal peptide and has a calculated molecular weight of 27.3kD. By SDS-PAGE, under reducing conditions, the apparent molecular mass is 40kD due to glycosylation. Recombinant protein corresponding to aa1-245 from human IFNGR1 extracellular ligand-binding domain, fused to His-Tag at C-terminal, expressed in HEK293 cells. Swiss/Uniprot: P15260 Molecular Weight: ~27.3kD Applications: Suitable for use in Western Blot and Cell-based assays. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Biological Activity: Measured by its ability to inhibit recombinant human IFN-γ-mediated protection of WISH cells infected with vesicular stomatitis virus. The ED50 for this effect is typically 2-8ug/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Dry Ice