mTOR

Size

£754.00

SKU:: 058638
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Peptide corresponding to GTTVPESIHSFIGDGLVKPE from human mTOR.
Format:: Supplied as a liqud in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide.
Extra Details:: Mammalian target of rapamycin (mTOR) is a serine/threonine kinase that plays a key role in protein synthesis, cell growth, proliferation and differentiation. It is inhibited by rapamycin, which has immunosuppressant functions. The 290kD protein is highly regulated by growth factors and nutrients like glucose and amino acids. mTOR is involved in several pathways from protein degradation via autophagy to protein synthesis via translation initiation (4EBP), ribosome and lipid synthesis. It is located in vesicles and the cytosol. Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, Western Blot and Immunoprecipitation. Knock out validated. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:2000. Immunocytochemistry/Immunofluorescence: 1:500-1:2000. Immunohistochemistry (paraffin): 1:50-1:100 (microwave treatment recommended). Immunoprecipitation: 10ug. Optimal dilutions to be determined by the researcher.. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice