058637
J-Chain (Immunoglobulin J Chain)
Size
£652.00
- SKU:
- 058637
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recominant protein corresponding to human J-Chain (Position: amino acids Q23-D159), expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, 4% trehalose. Reconstitute with 200ul sterile ddH2O.
- Extra Details:
- Immunoglobulin J chain, also known as IGJ and IGCJ, is a secreted polypeptide which is the first immunoglobulin-related polypeptide expressed during the embryogenesis and differentiation of B cells in the fetal liver. The joining Immunoglobulin J chain is a small polypeptide, expressed by mucosal and glandular plasma cells, which regulates polymer formation of immunoglobulin (Ig)A and IgM. Immunoglobulin J chain / IGJ serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA, it induces larger polymers. Immunoglobulin J chain / IGJ also helps to bind these immunoglobulins to the secretory component. J-chain incorporation into polymeric IgA (pIgA, mainly dimers) and pentameric IgM endows these antibodies with several salient features. Immunoglobulin J chain / IGJ is involved in creating the binding site for pIgR / SC in the Ig polymers, not only by determining the polymeric quaternary structure but also by interacting directly with the receptor protein. Both the immunoglobulin J chain / IGJ and the pIgR/SC are key proteins in secretory immunity. Applications: Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.25-0.5ug/ml. Immunohistochemistry (Paraffin): 2-5ug/ml by heat. Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 min is required for staining of formalin/paraffin sections. Immunocytochemistry/Immunofluorescence: 5ug/ml. Flow Cytometry (Fixed): 1-3ug/1x10e6 cells. ELISA: 0.1-0.5ug/ml. Optimal dilutions to be determined by the researcher. Positive Controls: Western Blot: rat small intestine tissue, mouse spleen tissue. Immunohistochemistry: human thyroid cancer tissue, human liver cancer tissue, rat colon tissue. Immunocytochemistry/Immunofluorescence: U2OS cells. Flow Cytometry: A431 cells. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice