058628
Microsomal Epoxide Hydrolase (EPHX1, Epoxide Hydratase, Epoxide Hydrolase 1, mEH)
Size
£767.00
- SKU:
- 058628
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human mEH.
- Clone:
- 1H9
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Microsomal epoxide hydrolase (mEH) is a member of the α/β-hydrolase family that catalyzes the hydrolysis of xenobiotic and endogenous epoxides to diols. It is a single polypeptide chain with the N-terminal region functioning as a membrane anchor and the C-terminus containing the catalytic residues. mEH is localized to the endoplasmic reticulum and ubiquitously expressed, but becomes pseudo-soluble under a variety of pathological conditions. mEH dissociates from the endoplasmic reticulum in neoplastic and preneoplastic liver cells and plasma levels of mEH are positively correlated with the metastasis of Kaposi’s sarcoma to the liver. Viral infection by hepatitis C or hepatitis A also induces pseudo-solubilization of mEH and the development of autoantibodies against mEH, which are associated with liver damage and disease progression. Applications: Suitable for use in Immunofluorescence and ELISA. Other applications not tested. Recommended Dilutions: ELISA: 1:1000 Immunofluorescence: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice