PlGF (Placental Growth Factor Vascular Endothelial Growth Factor-related Protein, Placental Growth Factor, PGF) (Biotin)

Size

£991.00

SKU:: 058610
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Ala21-Arg149 from human PlGF, expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
Extra Details:: Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra- and inter-chain disulfides are conserved among these growth factors. In their PDGF-like regions, VEGF and PlGF also share ~53% amino acid sequence similarity. The gene for PlGF has been mapped to chromosome 14. As a result of alternative splicing, at least two PlGF mRNAs encoding monomeric PlGF precursors containing 149 and 170 amino acid residues have been described. The expression of PlGF is not widespread, but has been detected in human umbilical vein endothelial cells, placenta, choriocarcinoma cell lines, and in renal cell carcinoma associated with angiogenesis. The PlGF proteins bind with high-affinity to Flt-1, but not to Flk-1/KDR. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (Detection): 2-8ug/ml using 058609 or 058611 as capture antibody. Western Blot: 0.1-0.4ug/ml using recombinant human PlGF. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice