Sclerostin (SOST), Recombinant, Rat, aa29-213, His-Tag

Size

£416.00

SKU:: 054960
Molecular Weight:: 24.7
Purity:: ≥90%.
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 0.01% sarcosyl, 5% trehalose, 1mM EDTA, 1mM DTT, Proclin 300. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex.
Extra Details:: Recombinant protein corresponding to aa29-213 from rat Sclerostin, fused to His-Tag at N-terminal, expressed in E. coli. Predicted Molecular Weight: 24.7kD Accurate Molecular Weight: 32kD Isoelectric Point: 10.1 Subcellular Location: Secreted Amino Acid Sequence: FKNDATEIIP GLREYPEPPQ ELENNQTMNR AENGGRPPHH PYDTKDVSEY SCRELHYTRF VTDGPCRSAK PVTELVCSGQ CGPARLLPNA IGRVKWWRPN GPDFRCIPDR YRAQRVQLLC PGGAAPRSRK VRLVASCKCK RLTRFHNQSE LKDFGPETAR PQKGRKPRPR ARGAKANQAE LENAY Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 . Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37ºC for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.

Shipping Conditions:: Blue Ice