Sclerostin (SOST), Recombinant, Mouse, His-Tag, GST-Tag

Size

£416.00

SKU:: 054959
Molecular Weight:: 50.7
Purity:: ≥95%.
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 0.01% sarcosyl, 5% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml. Do not vortex.
Extra Details:: Recombinant protein corresponding to aa25-209 from mouse Sclerostin, fused to His-Tag and GST-Tag at N-terminal, expressed in E. coli. Predicted Molecular Weight: 50.7kD Accurate Molecular Weight: 51kD Subcellular Location: Secreted Amino Acid Sequence: GWQAFRNDAT EVIPGLGEYP EPPPENNQTM NRAENGGRPP HHPYDTKDVS EYSCRELHFT RYVTDGPCRS AKPVTELVCS GQCGPARLLP NAIGRGKWWR PSGPDFRCIP DRYRAQRVQL LCPGGAAARA RKVRLVASCK CKRLTRFHNQ SELKDFGPET ARPQKGRKPR PRARSGKANQ AELEN Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37ºC for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.

Shipping Conditions:: Blue Ice