Desmoplakin 1/2

Size

£843.00

SKU:: 052909
Purity:: Supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to aa2115-2132 from human desmoplakin 2.
Clone:: DP447
Format:: Supplied as a liquid, 0.09% sodium azide.
Extra Details:: Desmoplakin (DP) is a cytoplasmic plaque protein that is located in desmosomes, which are abundant in tissues subjected to mechanical stress, like muscle and epidermis. The role of DP is to link the transmembrane cadherins via plakoglobin to the cytoplasmic intermediate filament network. DP comprises an N-terminal, desmosome-associated plakin domain binding to plakoglobin and plakophilin, a central coiled-coil rod domain responsible for dimerization, and a C-terminal domain consisting of three homologous plakin repeat subdomains, named “A”, “B”, and “C”, that interact with the intermediate filaments. Alternative splicing generates two isoforms (DP I and DP II) that differ in their rod domain lengths, with relative molecular weights of 332kD and 259kD, respectively. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence/Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Frozen/Paraffin): microwave treatment recommended for paraffin sections. Optimal dilutions to be determined by the researcher. Reactivity on Cultured Cell Lines: Several human carcinoma cell lines: MCF-7, A-431, HaCaT, CaCo2; bovine cells: MDBK, BMGE; RTG-2 (rainbow trout). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice