CD1d (Antigen-Presenting Glycoprotein CD1d, R3G1)

Size

£793.00

SKU:: 052898
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Pooled CD1d-GST fusion proteins containing α1 domain alone, α1-α2 domains, or α1–α3 domains.
Clone:: D5
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Extra Details:: Antigen-presenting glycoprotein CD1d (UniProt P15813; also known as CD1d, R3G1) is encoded by the CD1D gene (Gene ID 912) in human. CD1d is an MHC-like glycoprotein expressed on epithelial cells and can signal innate immune responses by presenting lipid antigen to natural killer (NK) and NK T (NKT) cells. Similar to MHC class I heavy chain (HC), CD1d HC is glycosylated and assembled with β2m in ER. Unlike MHC class I HC, misfoled CD1d and CD1d not properly assembled with β2m are not destroyed by ER quality control mechanisms. CD1d plays a role in both innate and adaptive immunity to various bacteria, viruses, fungi, and parasites. Activation of CD1d-restricted invariant NKT cells enhances host resistance to these microbes via direct killing of CD1d-expressing (infected) cells or by promoting interferon gamma release from conventional NK cells and by modulating adaptive immune cells via alteration of Th1/Th2 polarization. Chlamydia trachomatis is shown to prevent CD1d-mediated innate immune destruction of the infected urethral epithelial cells by targeting CD1d for proteolytic degradation. Specifically, the chlamydial proteasome-like activity factor (CPAF) interacts with the CD1d HC, and CPAF-associated CD1d HC is then ubiquitinated for eventual degradation. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Western Blot: 4ug/ml detected CD1d in 10ug of THP-1 cell lysate. Note: Longer exposure time is needed when using total lysates for Western blotting applications. Membrane preparation or immunoprecipitation can be employed to enrich membrane target proteins prior to Western blotting. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice