HHEX (Hematopoietically-Expressed Homeobox Protein, HEX, PRH, PRHX)

Size

£869.00

SKU:: 052885
Purity:: Purified by Protein A/G affinity chromatography from culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Thr111-Gly270 from human HHEX, expressed in E. coli.
Clone:: 2018B (recomb)
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Hematopoietically-expressed homeobox protein (HHEX), also known as HEX, PRH and PRHX, is a 35-40kD member of the Homeobox family of transcription factors. Family members are distinguished by an evolutionarily conserved DNA-binding homeodomain of 60 amino acids (aa), which for HHEX spans aa137-196. Human HHEX was initially isolated from hematopoietic tissue, and is present in several hematopoietic progenitors, where its expression is down-regulated during terminal cell differentiation. HHEX is also expressed in the anterior visceral endoderm during early mouse development, and in some adult tissues of endodermal origin, including liver, lung and thyroid. HHEX knockout in mice is embryonic lethal, with impaired forebrain, liver and thyroid development. Human HHEX is 270aa in length, and over aa111-270, shares 93% and 95% identity with mouse and rat HHEX, respectively. Applications: Suitable for use in Flow Cytometry, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 2-25ug/ml using immersion fixed K562 human chronic myelogenous leukemia cell line. Flow Cytometry: 0.25ug/10e6 cells in A549 human lung carcinoma cell line. Western Blot: 1ug/ml with lysates of HepG2 human hepatocellular carcinoma cell line, BaF3 mouse pro-B cell line, and H4-II-E-C3 rat hepatoma cell line under reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice