Estradiol (17b-Estradiol; 17beta-Estradiol)

Size

£723.00

SKU:: 052883
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: BSA-conjugate Estradiol (Estradiol-6-BSA).
Clone:: 6E1
Format:: Supplied as a liquid in PBS. No preservatives added.
Extra Details:: Estradiol is a major female sex hormone that regulates estrous and menstrual cycle. It is also present in smaller quantities in males where it is an active metabolic product of testosterone. In females, it is mainly produced by the granulosa cells in the follicles of ovaries. Smaller amounts of estradiol are also produced by the adrenal glands. It acts as an agonist for the estrogen receptor (ER) and can bind to both ERa and ERb. It is synthesized from cholesterol where androstenedione is a key intermediate which is converted to estradiol by aromatase. It is metabolized via hydroxylation into catechol estrogens. In the liver it can undergo hydroxylation into 2-hydoxyestradiol by CYP1A2, CYP3A4, and CYP2C9. Estradiol is reported to directly act on the central nervous system to modulate energy balance and reduced levels of estradiol following ovarian insufficiency have been linked to menopause, hyperphagia, and weight gain. Applications: Suitable for use in ELISA, Immunocytochemistry and Activity Assays. Other applications not tested. Recommended Dilution: Immunocytochemistry: 1:25 detected Estradiol (6-BSA) in OVCAR 3 cells. ELISA: Various titrations (2-fold serial dilutions) starting at 100ng/ml detected Estradiol (6-BSA). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice