AAV8 (Intact Particle)

Size

£895.00

SKU:: 052876
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: AAV8 capsids
Clone:: ADK8
Format:: Supplied as a liquid in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide.
Extra Details:: Adeno-associated virus (AAV) is a small virus which infects humans and some other primate species. AAV is not currently known to cause disease and consequently the virus causes a very mild immune response. AAV can infect both dividing and non-dividing cells and may incorporate its genome into that of the host cell. Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, Immunoprecipitation, Dot Blot, Neutralization and ELISA. Not for use in immunoblotting using denaturing conditions. Other applications not tested. Recommended Dilution: Dot Blot: 1:100-1:500 (0.1-0.5ug/ml; non-denaturing conditions). Neutralization Assay: EC50 ~14ng/ml (AAV3), ~4ng/ml (AAV8). Assay dependent. Optimal dilutions to be determined by the researcher. Binding Affinity: KD value (AAV8) = <1.0E-12 M. KD value (AAVrh10) = <1.0E-12 M. KD value (AAVrh74) = <1.0E-12 M. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.