052874
EED (Embryonic Ectoderm Development, hEED, HEED, Polycomb Protein EED, WAIT1, WAIT-1, WD Protein Associating with Integrin Cytoplasmic Tails 1)
Size
£960.00
- SKU:
- 052874
- Application:
- WB
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Full length recombinant fusion protein corresponding to mouse EED, tagged with MBP.
- Clone:
- AA19
- Format:
- Supplied as a liquid in 0.1M Tris-Glycine, pH7.4, 150mM sodium chloride, 0.05% sodium azide.
- Extra Details:
- Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:5000 detects EED on 15ug/ml of A431 cell lysate. Optimal dilutions to be determined by the researcher. Positive Control: A431 Cell Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice