p120 (Catenin, delta1)

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£876.00

SKU:: 052872
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Mouse p120 catenin (pY96)
Clone:: EBS-CA-011
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: The membrane associated protein pp120 Src substrate (p120 catenin, p120cas) was identified as a tyrosine kinase substrate that is phosphorylated in Src transformed cells or in response to growth factor stimulation. It shares structural similarity with the Drosophila Armadillo protein and the vertebrate beta-catenin and gamma-catenin proteins. Its characteristic Arm domain that is composed of 42-amino acid motif repeats evidences this similarity. In the cell, pl20 catenin is localized to the E-cadherin/catenins cell adhesion complex. Like beta- and gamma-catenin, p120 catenin directly associates with the cytoplasmic C-terminus of E-cadherin via its Arm domain and may similarly interact with other cadherins. It exists as four isoforms that range in size from 90-115kD. Expression of these isoforms is heterogeneous in human carcinomas, suggesting that altered pp120 expression contributes to malignancy due to loss of functional cell adhesions. Multiple tyrosine residues (Y96, Y112, Y228, Y280, Y257, Y291, Y296, and Y302) in p120 catenin are phosphorylated by Src and these phosphorylations may facilitate interaction with PTP1C/SHP-1 in response to EGF stimulation. Thus, p120 catenin is an Arm domain protein that interacts with both cell adhesion molecules, such as cadherins and cell signalling molecules, such as PTP1C. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (frozen): 1:50-1:100 (1-2ug/ml) Western Blot: 1:50-1:100 (1-2ug/ml) Optimal dilutions to be determined by the researcher. Positive Control: Carcinoma. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice