052869
M13 Phage
Size
£633.00
- SKU:
- 052869
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- M13 Bacteriophage
- Clone:
- MM05 (recomb)
- Format:
- Supplied as a liquid in PBS. No preservative added.
- Extra Details:
- M13 Bacteriophage is a filamentous F-pilus-specific bacteriophage (Ff phage), which is a long, thin virus that infects bacterial host cells. It is composed of circular single-stranded DNA (ssDNA) encapsulated by one major coat protein, p8, and four minor coat proteins, p3, p6, p7, and p9, and has approximately 98.5% sequence identity with other Ff phages such as f1 and fd. The p8 protein accounts for approximately 85% of the total virion mass, whereas p3, p6, p7, and p9, collectively account for approximately 3% of the total virion mass. Compared with f1 and fd phages, M13 bacteriophage coat proteins only differ by one amino acid at position 35 in the p8 protein. The M13 bacteriophage p8 protein is composed of an N-terminal segment that constitutes the external virion surface, a transmembrane segment, and a positively charged C-terminal segment that interacts with the ssDNA. M13 bacteriophage has commonly been used for molecular cloning, peptide phage display technology, microorganism detection, including Salmonella, cell imaging, and drug delivery. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:2500-1:10,000. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice