Claudin 6 (Skullin)

Size

£722.00

SKU:: 052865
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Extracellular peptides specific for human Claudin-6.
Clone:: 2
Format:: Supplied as a liquid in PBS. No preservative added.
Extra Details:: Claudin-6 (UniProt: P56747; also known as Skullin) is encoded by the CLDN6 (also known as UNQ757/PRO1488) gene (Gene ID: 9074) in human. Claudin-6 is a multi-pass membrane protein that plays a major role in tight junction-specific obliteration of the intercellular space. Claudin-6 has two extracellular domains (aa29-81 and 138-160), four transmembrane domains (aa8-28, 82-102, 117-137, and 161-181), and three cytoplasmic domains (aa1-7; 103-116, and 182-220). It acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells. Claudin-6 is expressed in the liver, in peripheral blood mononuclear cells, and hepatocarcinoma cell lines. It is also expressed specifically in undifferentiated stem cells and can serve as a marker to purify stem-like cells from a mixed cell population. It is shown to play an important role during mouse embryonic epithelium formation and the development of endodermal tissues, and its level of expression is correlated with dedifferentiation and malignant proliferation of epithelial cells. Applications: Suitable for use in Flow Cytometry, Immunocytochemistry, immunohistochemistry, Inhibition Assays and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:500 detected Claudin-6 in NTERA-2 cell lysate. Immunohistochemistry: paraffin sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice