PAD3 (PADI3, PDI3, Peptidylarginine Deiminase 3, Protein Arginine Deiminase 3)

Size

£869.00

SKU:: 052850
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Full length recombinant protein corresponding to human PAD3.
Clone:: 4E5
Format:: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
Extra Details:: Protein arginine deiminase 3 (PAD3) catalyzes the conversion of arginine residues to citrulline within its cellular protein substrates, which include S100A3, trichohyalin, and filaggrin, resulting in the loss of a positive charge, which can alter protein structure and/or function. It exists as a homodimer and is composed of a C-terminal catalytic domain and an N-terminal domain that contains two immunoglobulin G (IgG) subdomains. PAD3 is primarily expressed by differentiated keratinocytes in the epidermis and hair follicles where it has roles in the shaping and mechanical strengthening of hair. It is also expressed by neural stem cells where it modulates cell death by regulating the nuclear translocation of apoptosis-inducing factor (AIF), in mammary glands where it citrullinates proteins during lactation, and cancer cells where it promotes cancer cell invasion. Mutations in PADI3 are associated with uncombable hair syndrome. Applications: Suitable for use in Immunofluorescence, Western Blot and ELISA. Other applications not tested. Recommended Dilutions: ELISA: 1:1000 Western Blot: 1:00 Immunofluorescence: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice