GFAP, Citrullinated (R416)

Size

£850.00

SKU:: 052847
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Peptide corresponding to the C-terminal region of human GFAP citrullinated at R416.
Clone:: 4B8
Format:: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
Extra Details:: Citrullinated glial fibrillary acidic protein (citGFAP) is a citrullinated form of GFAP, a class III intermediate filament (IF) protein that is expressed in mature astrocytes and contributes to cytoskeletal structure and strength. GFAP can be citrullinated in a calcium-dependent manner on the arginine residue at position 270 (R270) and at R416 by protein arginine deiminase 1 (PAD1) and PAD2. citGFAP levels are increased by the calcium ionophore ionomycin in an in vitro model of traumatic brain injury (TBI) using NHA CC-2565 human astrocytes and are induced by controlled cortical impact in the cerebral cortex in a rat model of TBI. It has been found in the hippocampus in a rat model of Alzheimer's disease induced by amyloid-β (25-35), as well as in the retina in a mouse model of alkali-induced eye injury. citGFAP has also been found in postmortem hippocampus from patients with Alzheimer's disease, and citGFAP levels are increased in postmortem-derived brain lesions from patients with multiple sclerosis. Applications: Suitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 ELISA: 1:1000 Immunohistochemistry (FFPE): 1:200. Requires HIER with citrate buffer, pH 6.0. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice