052846-HRP
Vimentin, Citrullinated (HRP)
Size
£832.00
- SKU:
- 052846-HRP
- Application:
- WB
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to human vimentin with citrullines at 144 and 146 (G146R mutation)
- Clone:
- 12G11
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.05% ProClin™ 300, 50% glycerol and proprietary stabilizers Labeled with horseradish peroxidase (HRP).
- Extra Details:
- Vimentin is a cytoskeleton intermediate filament protein present in cells of mesenchymal origin, including leukocytes, endothelial cells, and smooth muscle cells. It is subject to citrullination by peptidyl arginine deiminase 2 (PAD2) under high calcium concentrations, which can occur during macrophage apoptosis. Citrullinated vimentin has been shown to have a role in the production of anti-citrullinated protein antibodies (ACPAs). Serum levels of immune complexes targeting citrullinated vimentin are increased in patients with rheumatoid arthritis and positive for ACPAs. Serum and plasma levels of citrullinated vimentin are also increased in patients with lung cancer or idiopathic pulmonary fibrosis, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice