052842-HRP
Citrulline (HRP)
Size
£850.00
- SKU:
- 052842-HRP
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Citrulline-containing peptide conjugated to KLH
- Clone:
- 1D9
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.05% ProClin300, proprietary stabilizers, 50% glycerol. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- Citrulline is a noncoding amino acid that is produced by deimination of arginine through the post-translational modification citrullination. Citrullination is catalyzed by protein arginine deiminases (PADs) that convert positively charged arginine to electrically neutral citrulline, decreasing the isoelectric point of the protein, altering the native protein structure, and influencing protein ionic interactions. Protein citrullination has roles in many physiological and pathological processes, including autoimmunity, cancer, and neurodegenerative disorders. Citrullination of arginine 26 on histone H3 by PAD2 displaces histone H3 from chromatin, resulting in chromatin decondensation and estrogen receptor α (ERα) transcriptional activation in a reporter assay. Citrullinated histones are also a component of neutrophil extracellular traps (NETs), a network of decondensed DNA and intracellular proteins secreted by neutrophils as a pathogen defense mechanism that is also a source of citrullinated autoantigens. Increased levels of antibodies to citrullinated protein antigens (ACPAs) are associated with increased disease severity in patients with rheumatoid arthritis. Plasma levels of citrullinated histone H3 are increased in patients with advanced cancer, and citrullinated glial fibrillary acidic protein (citGFAP) has been found in postmortem hippocampus from patients with Alzheimer's disease. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:1000 ELISA: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice