Yellow Fever Virus (Genome Polyprotein)

Size

£761.00

SKU:: 052836
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Yellow Fever Virus strain 17D.
Clone:: 2D12.A
Format:: Supplied as a liquid in PBS. No preservatives added.
Extra Details:: Yellow fever virus is an arbovirus of the Flavivirus genus. It is a single-stranded RNA virus, characterized by its spherical shape and an envelope derived from host cell membranes. The viral genome encodes a polyprotein (UniProt: P03314, Gene ID: 1502173) in Yellow fever virus (strain 17D), that is cleaved into structural proteins, including the capsid (C), surrounded by a lipid bilayer derived from the host cell, which contains two key envelope proteins: pre-membrane (prM), and envelope (E) proteins, essential for viral assembly and entry into host cells. The Envelope protein E plays a critical role in mediating fusion with host cell membranes, and its structure undergoes conformational changes upon exposure to neutral pH, facilitating viral entry. YFV is the cause of acute viral hemorrhagic disease transmitted by mosquitoes. YFV primarily infects hepatocytes, where it replicates and leads to cell death, contributing to the pathogenesis of yellow fever, a severe hemorrhagic disease fever, including fever, chills, loss of appetite, fatigue and in severe cases, liver failure, hemorrhage, and even death. Applications: Suitable for use in Inhibition Assays, ELISA, Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice