052835
mPTGES-1 (Prostaglandin E Synthase, EC:5.3.99.3, Microsomal Glutathione S-Transferase 1-like 1, MGST1-L1, Microsomal Prostaglandin E Synthase 1, MPGES-1, p53-induced Gene 12 Protein) (Not for Export EU)
Size
£735.00
- SKU:
- 052835
- Purity:
- Serum
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Full-length recombinant protein corresponding to human microsomal prostaglandin E synthase, fused to His-Tag. Species Sequence Homology: rat
- Format:
- Supplied as a liquid.
- Extra Details:
- Prostaglandin E synthase (UniProt: O14684; also known as EC:5.3.99.3, Microsomal glutathione S-transferase 1-like 1, MGST1-L1, Microsomal prostaglandin E synthase 1, MPGES-1, p53-induced gene 12 protein) is encoded by the PTGES gene (Gene ID: 9536) in human. mPGES -1 is a multi-pass membrane protein that is integral membrane, inducible and glutathione-dependent terminal synthase in the prostanoid biosynthesis pathway and acts downstream cyclooxygenases (COX) catalyzing the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). Functionally, mPGES-1 is coupled to COX-2 and the induction of these two enzymes by pro-inflammatory cytokines, growth factors or endotoxin leads to a surge of PGE2 synthesis by inflammatory cells. It is strongly expressed with COX-2 in synovial fibroblasts and macrophages. In cases of osteoarthritis, mPGES-1 and COX-2 are strongly expressed in the chondrocytes and the synovial lining cells. mPGES-1 also play a role in the pathogenesis of ischemic stroke and many neurodegenerative diseases. In Alzheimer disease brains higher expression of both COX-2 and mPGES-1 is observed. Higher expression of mPGES has also been reported in several cancers and this higher expression has been correlated with vascular invasion and worse prognosis in colorectal cancer. A549 and DU145 xenografts lacking mPGES-1 display significantly reduced growth rates. Applications: Suitable for use in Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:10,000 dilution of this antibody detected mPTGES-1 in recombinant microsomal Prostaglandin E synthase 1 protein. Immunohistochemistry (FFPE): 1:5000 detected mPTGES-1 in human tonsil and human placenta tissue. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice