SCYL1

Size

£819.00

SKU:: 052820
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: KLH-conjugated linear peptide corresponding to 16aa from the C-terminal region of human SCY1-Like Protein 1. Conserved between isoforms 1, 2, and 3.
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Extra Details:: N-terminal kinase-like protein (UniProt: Q96KG9; also known as Coated vesicle-associated kinase of 90 kDa, SCY1-like protein 1, Telomerase regulation-associated protein, Telomerase transcriptional element-interacting factor, Teratoma-associated tyrosine kinase) is encoded by the SCYL1 (also known as CVAK90, GKLP, NTKL, TAPK, TEIF, TRAP, HT019) gene (Gene ID: 57410) in human. SCY1-like protein 1 is ubiquitously expressed and it regulates COPI-mediated retrograde protein traffic at the interface between the Golgi apparatus and the endoplasmic reticulum. It is involved in the maintenance of the Golgi apparatus morphology. It has a divergent N-terminal kinase domain (aa 14-314), but it is catalytically inactive. It also contain three HEAT repeats (aa 350-388; 389-427; and 507-545). Six isoforms of SCY1-like protein 1 have been reported that are produced by alternative splicing. Its intracellular distribution is isoform specific. Isoforms 1 and 2 are shown to be cytoplasmic throughout the cell cycle. Isoform 3 is cytoplasmic during interphase and centrosomal during mitosis. Isoform six is generally localized to nucleus and it acts as a transcriptional activator and binds to three different types of GC-rich DNA binding sites (box-A, -B and -C) in the beta-polymerase promoter region. Mutations in SCYL1 gene are reported to cause Spinocerebellar ataxia, a clinically and genetically heterogeneous group of cerebellar disorders due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. Applications: Suitable for use in Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.2ug/ml detected SCY1-Like Protein 1 (SCYL1) in HeLa cell lysate. Immunocytochemistry: 1:100 detected SCYL1 in HeLa, A431, HUVEC, and NIH/3T3 cells. Optimal dilutions to be determined by the researcher Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice