Titin (NT/Z-Domain) (Connectin, TTN)

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£716.00

SKU:: 052815
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: GST-tagged recombinant protein corresponding to human Titin.
Clone:: 17D11.1
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Extra Details:: Titin is a large complex protein containing over one hundred IgG-like and fibronectin type-III domains, in addition to multiple Kelch, RCC1, TPR, and WD repeats. It is a member of the CAMK Ser/Thr protein kinase family and also possesses a protein kinase domain, which is involved in controlling gene expression and protein turnover in muscle tissues. Titin also functions as a sensor to mediate appropriate cellular responses to mechanical stress. In striated muscle tissue, titin regulates the length of the sarcomere by integrating myosin and actin filaments at the Z-disk and M-band, and facilitates the elasticity of the sarcomere. Titin also regulates sarcomere assembly and functions as central hub in signal transduction pathways. Defects in Titin may contribute to various forms of cardiomyopathy. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry (FFPE): 1:1000 detected Titin in human and mouse cardiac muscle tissues. Western Blot: 1:10-1:20 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice