Choline Acetyltransferase (CHAT)

Size

£806.00

SKU:: 052810
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Human placental enzyme. Species Sequence Homology: avian, chicken, guinea pig, monkey, rat, and opposum.
Format:: Supplied as a liquid in 5mg/ml BSA, 0.09% sodium azide.
Extra Details:: Choline acetyltransferase (ChAT) has a molecular weight of about 68kD. It is synthesized in the parakaryon and is transported to the axon terminals. ChAT belongs to the choline/carnitine acyltransferase family. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker. Choline acetyltransferase is found in cholinergic neurons in brain and spinal cord. Applications: Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000 detected CHAT on 10ug of mouse brain lysates. Immunohistochemistry (FFPE): 1:100 Optimal dilutions to be determined by the researcher. Positive Control: Human placenta lysates, rat forebrain/rostral hypothalamus. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.