052805
p67 phox (NCF2, Neutrophil Cytosol Factor 2, 67kD Neutrophil Oxidase Factor, NCF-2, Neutrophil NADPH Oxidase Factor 2, NOXA2)
Size
£678.00
- SKU:
- 052805
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- KLH-conjugated linear peptide corresponding to the N-terminus of mouse p67-phox.
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
- Extra Details:
- Neutrophil cytosol factor 2 (UniProt Q09014; also known as 67 kDa neutrophil oxidase factor, NADPH oxidase activator 2, NADPH oxidase subunit 67 kDa, NCF-2, Ncf2, Neutrophil NADPH oxidase factor 2, p67-phox, p47phox) is encoded by the murine Ncf2 (also known as Noxa2, p67phox) gene in murine species (Gene ID 17970). Activation of NADPH-oxidase dependent superoxide generation requires the participation of several cytosolic factors, including p47-phox, p67-phox, p40-phox and Rac. In the resting cell p47phox, p67phox, and p40phox form a large molecular weight cytosolic complex. Activation of the NADPH oxidase is initiated by the assembly of p47phox, p67phox, and Rac with cytochrome b558 in a 1:1:1:1 complex in the plasma membrane. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml detected p67-phox (rodent) in 10ug of C6 and THP-1 cell lysate. Immunohistochemistry (FFPE): 1:50 detected p67-phox (rodent) in rat colon and mouse hindbrain tissue. Requires heat-induced epitope retrieval (HIER) Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt . For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice