ABCA1 (ATP-Binding Cassette Sub-family A Member 1, ATP-Binding Cassette Transporter 1, ATP-Binding Cassette 1, ABCA 1, ABCA-1)

Size

£754.00

SKU:: 052801
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Linear peptide corresponding to the C-terminus of mouse ABCA1.
Clone:: MABI98-7
Format:: Supplied as a liquid in PBS, 0.05% sodium azide.
Extra Details:: ATP-binding cassette sub-family A member 1, also known as ATP-binding cassette transporter 1 (ABC-1), or ATP-binding cassette 1, and encoded by the gene name Abca1 or Abc1, is a cAMP-dependent and sulfonylurea-sensitive anion transporter. ABCA1 is a member of the ABC transporter superfamily and the ABCA family. ABCA1 is a key gatekeeper influencing intracellular cholesterol transport. ABCA1 has two homologous halves, each containing a hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain, and may act as a multifunctional polypeptide. ABCA1 has been shown to be down-regulated by endotoxins (LPS) or cytokines (TNF and IL-1) in J774 macrophages. The down-regulation by endotoxin in macrophages is not likely to be mediated by the liver X receptor/retinoic X receptor (LXR/RXR). ABCA1 interacts with MEGF10 and is widely expressed in adult tissues, with the highest levels found in uterus and pregnant uterus. Mutations in the ABCA1 gene are associated with Tangier disease (TD). TD is an autosomal recessive disorder results from an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and disorders in cellular lipid trafficking. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry (FFPE): 1:250 detected ABCA1 in human cerebral cortex, thalamus, and kidney tissues. Requires HIER. Western Blot: 1ug/ml detected ABCA1 in 10ug of Calyculin and Okadaic Acid treated NIH-3T3 cell lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice