TAPA-1 (CD81, Tetraspanin-28, Tspan-28)

Size

£761.00

SKU:: 052796
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: OCI-LY8 whole cells (Cellosaurus cell line).
Clone:: 5A6
Format:: Supplied as a liquid in PBS. No preservative added.
Extra Details:: CD81 antigen (UniProt: P60033; also known as 26kD cell surface protein TAPA-1, Target of the antiproliferative antibody 1, Tetraspanin-28, Tspan-28, CD81) is encoded by the CD81 (also known as TAPA1, TSPAN28) gene (Gene ID: 975) in human. TAPA-1 is a nonglycosylated, homodimeric, multi-pass membrane protein of the tetraspanin (TM4SF) family. It is expressed in a wide range of cells, including hematolymphoid, neuroectodermal, and mesenchymal tumor cell lines and is reported to play a role in the regulation of lymphoma cell growth. It contains four transmembrane domains, two extracellular loops, and short amino and carboxyl termini that are intracellular. TAPA-1 is shown to affect cell adhesion, morphology, activation, proliferation, and differentiation of B, T, and other cells. On B cells it is a part of a complex involving CD21, CD19, and Leu13 and this complex is reported to reduce the threshold for B cell activation via the B cell receptor by bridging antigen specific recognition and CD21-mediated complement recognition. On T cells, it is shown to associate with CD4 and CD8 and provides a costimulatory signal with CD3. It is also reported to act as a receptor for hepatitis C virus (HCV) in hepatocytes. Mutations in CD81 gene cause common variable immunodeficiency 6 (CVID6) that is characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections, and an inability to mount an antibody response to antigen. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Inhibition assays. Other applications not tested. Recommended Dilution: Flow Cytometry: 1ug detected TAPA-1 (CD81) in 1x10e6 HUH-7 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice