Sodium/Hydrogen Exchanger 3 (NHE-3, Solute Carrier Family 9 Member 3)

Size

£728.00

SKU:: 052795
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: MBP-conjugated recombinant protein fragment corresponding to 356aa from the C-terminal half of Opossum sodium/hydrogen exchanger 3. Species Sequence Homology: porcine (100%).
Clone:: 3H3
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: Sodium/hydrogen exchanger 3 (UniProt: Q28362; also known as Na(+)/H(+) exchanger 3, NHE-3, Solute carrier family 9 member 3) is encoded by the SLC9A3 (also known as NHE3) gene in Didelphis virginiana (North American opossum). NHE-3 is a multi-pass membrane protein that is involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. NHE-3 is predominantly expressed in kidney and gut, where it is found on the apical membranes of polarized epithelial cells and plays a central role in transepithelial reabsorption of sodium ions and secretion of hydrogen ions. Renal NHE-3 is essential in maintaining blood pressure and steady-state plasma sodium levels when dietary sodium chloride intake is modified. In the intestine epithelial cells, it localizes to the ileal brush border. Dysfunction of NHE-3 in intestine is associated with a variety of diarrheal diseases. HNE-3 can be phosphorylated by SGK1 at Ser669 that increases its abundance at the cell membrane. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (FFPE): 1:50 detected NHE3 in human kidney and human stomach tissue sections. Western Blot: 0.25ug/ml detected NHE3 in lysates from COS-7 cells transfected with wild type NHE3 plasmid DNA. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice