Surfactant Protein B (SP-B)

Size

£754.00

SKU:: 052793
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: GST-tagged recombinant protein corresponding to human Surfactant Protein B at the Saposin B-type 2 domain.
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: Surfactant Protein B (SP-B) is mostly composed of phospholipids, but also incorporates essential proteins. It is necessary for pulmonary surfactant function and the alveolar type 2 cell phenotype. SP-B deficiency has been known to cause congenital pulmonary alveolar proteinosis. SP-B is thought to protect lungs against endotoxin-induced inflammation. SP-B could be used as a clinical marker in chronic heart failure. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (paraffin): 1:200 detected Surfactant protein B in clara cells in normal rat lung tissue. Western Blot: 1:5000 detected Surfactant Protein B on 10ug of human lung tissue lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.