052790
HUR, phosphorylated (Ser221) (ELAVL1, ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-like 1 (Hu Antigen R), ELAV1, ELAV-like Protein 1, Hua, Hu-antigen R, HuR, MelG)
Size
£735.00
- SKU:
- 052790
- Application:
- WB
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- KLH-conjugated linear peptide corresponding to human phospho HuR (Ser221). Species Sequence Homology (100%): mosue, porcine, bovine, chicken and xenopus.
- Format:
- Supplied as a liquid in Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
- Extra Details:
- HuR , also known as Hu-antigen R, ELAV-like protein 1, and encoded by the gene name ELAVL1 and HUR, is a member of the RRM ELAV family. HuR is an RNA bindnig protein that contains 3 RNA-binding domains. It is known to binds AU-rich elements if mRNA. It regulates gene expression by destabilizing their mRNA. Recent studies have shown that HuR is upregulated in certain cancers such as breast and gastric cancers. HuR is also involved in embryonic stem cells (ESCs) differentiation. HuR preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, and thus promoting ESCs differentiation. Additionally, HuR has been shown to bind to poly-U elements and AU-rich elements (AREs) in the 3′-UTR of target mRNAs, as well as the AU-rich element in FOS and IL3/interleukin-3 mRNAs. Some of HuR’s targets include cFos, IL-3, and BRCA1 mRNAs. HuR is also involved in the regulation of VEFG-A and NFkB expression in certain cell types. HuR activation and accumulation are also linked to many energy signaling molcules such as that of the PI3K/Akt pathway and AMPK. Applications: Suitable for use in Western Blot and Peptide Inhibition. Other applications not tested. Recommended Dilutions: Western Blot: 1:200 detected phospho HuR (Ser221) in 10ug of HCT116 cell lysate. Peptide Inhibition Analysis: 1:200 blocked phospho HuR (Ser221) in HCT116 cell lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice