Tau, Cleaved (D421) (Ascites Free)

Size

£767.00

SKU:: 052782
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: KLH-conjugated linear peptide corresponding to a human Tau sequence with Asp421 at the C-terminus.
Clone:: Tau-C3
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: Microtubule-associated protein tau (UniProt P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (also known as TAU, MAPT1, MTBTL) gene (Gene ID 4137) in human. Extracellular plaque deposits composed of amyloid-β and intracellular neurofibrillary tangles (NFTs) composed of truncated and hyperphosphorylated tau are two neuropathological hallmarks of Alzheimer′s disease (AD). Tau pathology (tauopathy) can cause toxicity when the brain is devoid of amyloid plaques, and tangle pathology correlates better with clinical dementia than amyloid pathology. Abnormal processing of tau by hyperphosphorylation and proteolytic truncation contribute to the toxicity of tau. Tau is known to be cleaved by various proteases, including caspases, calpains, cathepsins, thrombin, and puromycin-sensitive aminopeptidase, as well as the lysosomal cysteine proteinase asparagine endopeptidase (AEP). MAPK, GSK-3, and Cdk-5 are three known kinases that target various tau phosphorylation sites. Tau also undergoes other types of posttranslational modifications, including glycosylation, ubiquitination, glycation, polyamination, nitration, and lysine methylation, which are believed to be important for its non-pathological functions, including polymerization and stabilization of microtubules. Variable, depending on the Tau isoforms where the fragments are derived from and whether the fragements were cleaved at the N-terminus by other proteases. Uniprot P10636 lists 9 human tau isoforms produced by alternative splicing. The Asp421/D421 designation was based on D421 of isoform 8 (Tau-F, Tau-4), which is equivalent to D738 of isoform 1 (PNS-tau), D332 of isoform 2 (Fetal-tau), D296 of isoform 3 (Tau-A), D361 of isoform 4 (Tau-B), D390 of isoform 5 (Tau-C, Tau-3), D363 of isoform 6 (Tau-D), D392 of isoform 7 (Tau-E), and D756 of isoform 9 (Tau-G). Applications: Suitable for use in Immunocytochemistry, Immunofluorescence, Western Blot and Immunhistochemsitry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (FFPE): 1:250 detected Asp421-cleaved Tau in neurofibrillary tangles (NFTs) of Alzheimer′s diseased (AD) human brain tissue. Requires heat-induced epitope retrieval (HIER). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice