Neuropilin-2a, Extracellular Domain. Recombinant, Human, aa23-855, His-Tag

Size

£767.00

SKU:: 052758
Molecular Weight:: 95
Purity:: ≥95% (SDS-PAGE). Endotoxin: ≤1EU/ug (LAL).
Storage Conditions:: -80°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from sterile PBS, pH 7.4. Reconstitute with sterile PBS.
Extra Details:: Neuropilin-2 (NRP-2) is a non-tyrosine kinase transmembrane glycoprotein co-receptor for class III semaphorins and VEGFs.1 It is composed of two CUB domains (a1 and a2), two Factor V/VIII homology domains (b1 and b2), a MAM domain, a transmembrane region, and a cytoplasmic tail. The a1, a2, and b1 domains are responsible for binding to class III semaphorins, including semaphorin-3C (Sema3C) and Sema3F, the b1 and b2 domains are responsible for binding to VEGFs, including VEGFA165, VEGFA145, VEGFC, VEGFD, and placenta growth factor (PlGF), and the MAM domain is important for homo- or heterodimerization.1,2 NRP-2 exists in three main isoforms: membrane-bound NRP-2a and NRP-2b, which are generated by alternative splicing and differ by the presence or absence, respectively, of the PDZ binding motif in the C-terminus, and soluble NRP-2 (sNRP-2), which is composed of the a1, a2, and b1 domains and a partial b2 domain.1 NRP-2a is expressed in the brain, heart, kidney, liver, lungs, placenta, trachea, and small intestine.3 NRP-2 is a co-receptor for VEGFR2 and VEGFR3 and is involved in angiogenesis, lymphangiogenesis, and neuronal guidance, as well as some immune functions such as T cell migration.2,4,1 Knockout of Nrp2a induces misprojections of axons originating from olfactory sensory neurons expressing transient receptor potential canonical 2 (TRPC2) in zebrafish.4 High tumoral expression of NRP2A is associated with poor overall survival in patients with muscle-invasive bladder cancer.5 This protein consists of 844 amino acids, has a calculated molecular weight of 95kD, and a predicted N-terminus of Gln23 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is 100-110kD due to glycosylation. Active recombinant protein corresponding to aa23-855 from human Neuropilin-2a extracellular domain, fused to a C-terminal His-Tag, expressed in HEK293 cells. Swiss Prot: O60462 Molecular Weight: ~95kD Specific Activity: As Reported Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -80°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -80°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice