052725
Green Fluorescent Protein (GFP, GFPuv) (APC)
Size
£895.00
- SKU:
- 052725
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Ser2-Lys238 from GFPuv, expressed in E. coli.
- Clone:
- 25H34
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Extra Details:
- Green fluorescent protein (GFP) is a 27kD protein originally isolated from the jellyfish Aequorea victoria. In the presence of UV light (490-520nm), it emits a green fluorescent color that can be used to pinpoint locations of various intracellular proteins. GFP is 238 amino acids (aa) in length. It is a globular monomer that has a tendency to dimerize. The monomer has the shape of a beta -barrel with a chromophore (aa65-67) containing alpha -helix running up its center. GFPuv is the Aequorea sequence with three aa substitutions; Phe to Ser at amino acid 99, Met to Thr at 153, and Val to Ala at 163. This form expresses faster and is 18-fold brighter than native GFP; excitation peaks at 395nm and emission at 508nm. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul to label 10e6 cells using HEK293 human embryonic kidney cell line using intracellular staining. Optimal dilutions to be determined by the researcher. Absorption: 620-650nm Emission: 660-670nm Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice