052724
GFP (Green Fluorescent Protein)
Size
£882.00
- SKU:
- 052724
- Purity:
- Ascites
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- GFP tagged fusion protein.
- Clone:
- GFP-20
- Format:
- Supplied as a liquid, 0.09% sodium azide.
- Extra Details:
- GFP is a 27kD (238 amino acid) protein, derived from the bioluminescent jellyfish Aequorea victoria, in which light is produced when energy is transferred from the Ca2+-activated photoprotein aequorin to GFP. GFP is a unique tool to monitor dynamic processes in a variety of living cells or organisms. When expressed in either eukaryotic or prokaryotic cells and illuminated by blue or UV light, GFP yields a bright green fluorescence. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. Exogenous substrates and cofactors are not required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP signals can be quantified by flow cytometry, confocal scanning laser microscopy, and fluorometric assays. Indeed, many recombinant proteins have been engineered with GFP tags to facilitate the detection, isolation and purification of the proteins. The Green Fluorescent Protein (GFP) is derived from the jellyfish Aequorea Victoria and is often used as a gene expression marker. Thus, antibodies to GFP can facilitate the detection and analysis of GFP labeled nucleic acids and proteins. Applications: Suitable for use in Western Blot, Dot Blot and Indirect ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:2000 using purified recombinant GFP preparation Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice