Creatine Kinase BB (CK-BB), Porcine

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£698.00

SKU:: 052721
Storage Conditions:: -20°C
Supplier:: United States Biological
CAS Number:: 9001-15-4
Extra Details:: Creatine Kinase BB is concentrated in the brain and lungs. Because the CKBB isoenzyme, CPK-1 isoenzyme is predominately found in the brain and lungs, injury to either of these organs (for example, stroke or lung injury due to a pulmonary embolism) are associated with elevated levels of this isoenzyme. Source: Porcine Brain E.C. Number: 2.7.3.2 CAS No: 9001-15-4 Activity: ≥100U/mg Specific Activity: Reported in U/mg protein Unit Definition: One Unit will transfer one micromole of phosphate from creatine phosphate to ADP/minute at 37°C. Measured at 340nm as one equimolar amount of NADH produced by a coupled reaction. Protein: ≥0.6mg protein/mg (Coomassie) Isoenzyme Content: CK-BB: >95% CK-MB: <3% CK-MM: <3% Form: Supplied as a lyophilized powder from from Tris-succinate, DTT, EDTA, pH 6.5. Solubility (1mg/ml in saline): Clear, light yellow solution Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile saline. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.